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Just one challenge in the cross-examine comparability of qPCR-primarily based quantitative microbiome profiling is definitely the reliance on an exterior qPCR conventional from a reference organism required to construct an ordinary curve. In concept, any common taxon current inside a microbial community of curiosity can be employed since the reference organism for standard curve development.

In summary, we caution towards the Examination of microbiome NGS knowledge solely relying on relative abundance, considering that compositionality may possibly skew Organic inferences from microbiome scientific studies per

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